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A 54-year-old woman sees her primary practitioner for a routine, preventative healthcare visit. She has never previously had an HIV test. As part of routine HIV screening, blood is drawn for a laboratory-based HIV-1/2 antigen-antibody immunoassay using the Centers for Disease Control/Association of Public Health Laboratories (CDC/APHL) HIV testing algorithm.

Which one of the following statements is TRUE regarding laboratory-based HIV-1/2 antigen-antibody immunoassays?

The laboratory-based HIV-1/2 antigen-antibody immunoassays have the ability to detect HIV p24 antigen, HIV-1 antibodies, and HIV-2 antibodies. For most, if the test is positive, there is a simple color change in the well and it does not indicate which component caused the reactivity.
Figure 1. HIV-1/2 Antigen-Antibody Immunoassays
The laboratory-based HIV-1/2 antigen-antibody immunoassays have the ability to detect HIV p24 antigen, HIV-1 antibodies, and HIV-2 antibodies. For most, if the test is positive, there is a simple color change in the well and it does not indicate which component caused the reactivity.
Illustration: David H. Spach, MD
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Question Last Updated
January 28th, 2025

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